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A1. Nonmotile algae without chloroplasts:
Cyanobacteriophyta
A2. Motile algae with chloroplasts and flagella
A3. Nonmotile algae with chloroplasts but without flagella
A4. Cells with paired, symmetric semi-cells:
Desmids
B1. Cells rarely solitary; usually forming clumps or filaments
B2. Cells mostly solitary unless dividing
C1. Cells lack an obvious sinus; semi-cells usually separated by a simple suture that may not be visible
D1. Cells crescent-shaped, strongly or weakly curved, usually with tapered apices; cell wall lacking spines or teeth, but often striated; apex with vacuole containing one or more granules:
Closterium
D2. Cells spherical, oval, or broadly elliptical, with no obvious sinus
D3. Cells elliptical, spindle-shaped, or cylindrical
C2. Cells with relatively distinct sinus; sinus may be open or closed, deep or shallow
A5. Cells enclosed in glassy, ornamental frustules:
Diatoms