A1. Nonmotile algae without chloroplasts: Cyanobacteriophyta
A2. Motile algae with chloroplasts and flagella
A3. Nonmotile algae with chloroplasts but without flagella
A4. Cells with paired, symmetric semi-cells: Desmids
B1. Cells rarely solitary; usually forming clumps or filaments
B2. Cells mostly solitary unless dividing
C1. Cells lack an obvious sinus; semi-cells usually separated by a simple suture that may not be visible
C2. Cells with relatively distinct sinus; sinus may be open or closed, deep or shallow
D1. Cells with toothed whorls or horn-like lateral extensions along entire lateral margin; cells narrowly or broadly cylindrical, with an indistinct sinus
D2. Cells circular, oval, or broadly elliptical in cross-section
D3. Cells moderately to strongly flattened in cross-section; cells circular, oval, or broadly elliptical in outline
D4. Cells biradiate, triradiate, or polyradiate (more than 3 angles) in cross-section, usually distinctly angular
A5. Cells enclosed in glassy, ornamental frustules: Diatoms